| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Columbia Premier SC VUSA 16B WOLVERINES |
n/a | Jaclyn Vargas |
| A2 | Columbia Premier SC VUSA 16B STRIKERS |
n/a | Robert Tackwell |
| A3 | Columbia Premier SC VUSA 16B RAPTORS |
n/a | Felix Ayala |
| A4 | Columbia Premier SC VUSA 16B Super Timbers |
n/a | Thomas Guffey |
| A5 | Columbia Premier SC VUSA 16B Czajka |
n/a | Phillip Czajka |
| A6 | Columbia Premier SC CWSA 16B Cosmos |
n/a | Brian Monnin |
| A7 | Columbia Premier SC CWSA 16B Fresh |
n/a | Ronald Fresh |
| A8 | Columbia Premier SC CWSA 16B Galaxy |
n/a | Jessica Seay |
| A9 | Columbia Premier SC CWSA 16B Blue Lightning |
n/a | Daniel Carlson |
| A10 | Columbia Premier SC CWSA 16B Silly Heads |
n/a | Lindsay Bigby |
| A11 | Columbia Premier SC CWSA 16B Team 07 |
n/a | Omera Perdew |
| A12 | Columbia Premier SC CWSA 16B Blue Rays |
n/a | Matthew Richardson |